In vivo and in vitro hamster models in the assessment of virulence of recombinant influenza viruses
Identifieur interne : 002593 ( Main/Exploration ); précédent : 002592; suivant : 002594In vivo and in vitro hamster models in the assessment of virulence of recombinant influenza viruses
Auteurs : A. W. Heath [Royaume-Uni] ; Caroline Addison [Royaume-Uni] ; M. Ali [Royaume-Uni] ; D. Teale [Royaume-Uni] ; C. W. Potter [Royaume-Uni]Source :
- Antiviral Research [ 0166-3542 ] ; 1983.
English descriptors
- Teeft :
- Attenuated, Attenuated strains, Attenuated virus strains, Attenuation, Ciliary, Ciliary activity, Clone, Gesamte virusforsch, Hamster, Hamster lungs, Hamster tracheal organ cultures, Infant rats, Influenza, Influenza virus, Influenza virus infection, Influenza viruses, Inoculated, Lung extracts, Maassab, Maximum titres, Newborn rats, Organ cultures, Peak titres, Present study, Recombinant, Recombinant strains, Recombinant viruses, Titre, Tracheal, Turbinate, Turbinate extracts, Vaccine, Virol, Virulence, Virulent, Virulent strains, Virus, Virus attenuation, Virus inoculation, Virus strains, Virus titres, Virus vaccines, Virus virulence, Volunteer studies.
Abstract
Abstract: The virulence of five wild-type influenza A viruses and 14 recombinant viruses, prepared from the cold adapted A/Ann Arbor/6/60 virus and various wild-type viruses, was studied by two methods. Firstly, the viruses were inoculated into hamsters, and the titres present in the lungs and turbinates at 1, 3 and 4 days post-infection were measured. Secondly, the effect of five wild-type and ten recombinant viruses on the ciliated epithelium of in vitro hamster tracheal organ cultures was examined. The results obtained were assessed with reference to the known virulence of the viruses for human volunteers. The results showed that virus strains virulent for man grew to higher titres in hamster lungs and turbinates than attenuated strains; and that virulent strains destroyed the ciliary activity of hamster tracheal organ cultures more quickly and to a greater extent than attenuated strains. Comparison of the results with the known virulence of viruses tested for man suggests that the reduced ability of virus to grow in hamster lung tissue and the relatively little effect on ciliary activity may be used as markers of virus attenuation; however, the growth of virus in hamster turbinates overlaps for virulent and attenuated strains and therefore was not considered a useful marker of virulence.
Url:
DOI: 10.1016/0166-3542(83)90003-7
Affiliations:
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Potter</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Virology, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2 RX</wicri:regionArea><wicri:noRegion>Sheffield S10 2 RX</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Antiviral Research</title><title level="j" type="abbrev">AVR</title><idno type="ISSN">0166-3542</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">3</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="241">241</biblScope><biblScope unit="page" to="252">252</biblScope></imprint><idno type="ISSN">0166-3542</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-3542</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Attenuated</term><term>Attenuated strains</term><term>Attenuated virus strains</term><term>Attenuation</term><term>Ciliary</term><term>Ciliary activity</term><term>Clone</term><term>Gesamte virusforsch</term><term>Hamster</term><term>Hamster lungs</term><term>Hamster tracheal organ cultures</term><term>Infant rats</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus infection</term><term>Influenza viruses</term><term>Inoculated</term><term>Lung extracts</term><term>Maassab</term><term>Maximum titres</term><term>Newborn rats</term><term>Organ cultures</term><term>Peak titres</term><term>Present study</term><term>Recombinant</term><term>Recombinant strains</term><term>Recombinant viruses</term><term>Titre</term><term>Tracheal</term><term>Turbinate</term><term>Turbinate extracts</term><term>Vaccine</term><term>Virol</term><term>Virulence</term><term>Virulent</term><term>Virulent strains</term><term>Virus</term><term>Virus attenuation</term><term>Virus inoculation</term><term>Virus strains</term><term>Virus titres</term><term>Virus vaccines</term><term>Virus virulence</term><term>Volunteer studies</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The virulence of five wild-type influenza A viruses and 14 recombinant viruses, prepared from the cold adapted A/Ann Arbor/6/60 virus and various wild-type viruses, was studied by two methods. Firstly, the viruses were inoculated into hamsters, and the titres present in the lungs and turbinates at 1, 3 and 4 days post-infection were measured. Secondly, the effect of five wild-type and ten recombinant viruses on the ciliated epithelium of in vitro hamster tracheal organ cultures was examined. The results obtained were assessed with reference to the known virulence of the viruses for human volunteers. The results showed that virus strains virulent for man grew to higher titres in hamster lungs and turbinates than attenuated strains; and that virulent strains destroyed the ciliary activity of hamster tracheal organ cultures more quickly and to a greater extent than attenuated strains. Comparison of the results with the known virulence of viruses tested for man suggests that the reduced ability of virus to grow in hamster lung tissue and the relatively little effect on ciliary activity may be used as markers of virus attenuation; however, the growth of virus in hamster turbinates overlaps for virulent and attenuated strains and therefore was not considered a useful marker of virulence.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Heath, A W" sort="Heath, A W" uniqKey="Heath A" first="A. W." last="Heath">A. W. Heath</name></noRegion><name sortKey="Addison, Caroline" sort="Addison, Caroline" uniqKey="Addison C" first="Caroline" last="Addison">Caroline Addison</name><name sortKey="Ali, M" sort="Ali, M" uniqKey="Ali M" first="M." last="Ali">M. Ali</name><name sortKey="Potter, C W" sort="Potter, C W" uniqKey="Potter C" first="C. W." last="Potter">C. W. Potter</name><name sortKey="Teale, D" sort="Teale, D" uniqKey="Teale D" first="D." last="Teale">D. Teale</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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